how calibrate the hplc detector
Answer Posted / samba siva reddy.p
detector checking with caffiene soln bcz caffiene have 2 max and 1 minima wavelenths,we inject std stock soltn(caffiene soln) with diffrent wavelengths.
201 to 209= 205(maxima)
241 to 249=245(minima)
269 to277=275(maxima)
limits+ or -2
Is This Answer Correct ? | 2 Yes | 0 No |
Post New Answer View All Answers
what is the principle involved in turbidometry and nephelometry ?
How to set analyticl specification for combination products?
What is gelatinization?
What is the difference between purge septum flow and column flow in gas chromatography?
How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?
if you have given one product then how you determine the impurity in that?
What is shaking level in GC?
why we use glass fiber filters use in some situation?
How can the GC or HPLC method is selected to determine the impurity profile in drug product?
why we use Dichlorobenzene.nitrobezine.t-butyldi sulhate.for calibration of gc Head space
before starting analytical method valodation what you checking? and how giving preference to start validation?
how pda detector works over uv?
in gas chromatography what is the difference between gas flow rate and average linear velocity ?
what are risk assement in the analytical qbd?