Interested to Buy Any Domain ? << Click Here >> for more details...
Answer Posted / vinod kumar uppalli
The Si-OH functional groups of silica bed tend to ionize at higher pH and convert into negatively charged Si-O functional group and this interacts with acidic moieties and leads to tailing of peak due to secondary interactions. To overcome this most of the columns are end capped (Si-OH GROUP IS SHIELDED) to avoid secondary interactions.
| Is This Answer Correct ? | 1 Yes | 0 No |
Post New Answer View All Answers
on the basis of bcs class how to proceed the solubility?
What is importance of pH in the Heavy metal test and How it effect on the test results?
How do we fix the sample concentaryion in hplc method development. What is the basis?
Why to use propyl paraben in HPLC gradient calibration
In which situation we require to analytical method validation of excipient?
How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?
As per ICH related substances stability trend limit from initial to shelf life
what are the standard methods for HPLC?
What is similarity factor and its use in api strength?
all types of questions asked at the cipla interview
how to calculate elemental impurities?
what is biorelivent dissolution media?
how you start RS method development when for new product?
What is the use of tlc and hplc? And when and where use?
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
