Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

why 0.005 M sulphuric acid is used to calbrate
spectrophotometer and why caffiene is used for calibrating
hplc

Answer Posted / sp goswami

A 0.005M sufuric acid solution gives the required ionic
strength, required dissolution and also do not interfere in
UV-VIS Region giving 99% absorbance for the analyte of
interest so 0.005 M diluted sulfuric acid is useful .
Caffeine is a primary std ,pure form ,good absorbance and
readily available in market
Regards

Is This Answer Correct ?    10 Yes 3 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is diffrence between extractable volume and deliverable volume? Answer pls

4618


why we are using hexane in calibration of number of drop per mL

1792


What is the use of tlc and hplc? And when and where use?

1211


If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately

907


WHY given much noise PDA dector then VWD Dector

2144


in DMF having extra impurities and in api COA also having extra imp than USP or BP product then how require to proceed?

887


how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,

2652


why sre you used Potassium hydrogen phthalate in standarisation of 1N NaOH and 0.1 N Perchloric Acid?

9886


How to calibration of the uv spectroscopy and its test?

1242


For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?

1963


why we use Dichlorobenzene.nitrobezine.t-butyldi sulhate.for calibration of gc Head space

2331


Why are use silicon oil mr?

1257


what is the principle of UV Vis spectroscopy, AAS, ICP OES,ICPAES, ICP-MS and FTIR

9298


if your product is soluble in 0.1n hcl and water then which you choose as media from these 2 media?

735


can i use hplc detector to uplc and why?

2347