Answer Posted / bhagavanji nelakanti
Masking of secondary silanol groups of bonding phase by tri
methyl silane (TMS)molecules is called as end capping. In
the bonded phase the secondary silanol molecules which shows
high polar bonding property with compound retains in the
column and those compounds elute slowly. Because of the end
capping the binding property of the secondary silanol
molecules will reduce and resulting less retention of those
compounds.
| Is This Answer Correct ? | 45 Yes | 3 No |
Post New Answer View All Answers
What is the principle of HPLC, GC, LCMS, GCMS,LC QQQ, GC QQQ, LC Q TOF and GC Q TOF. What are the applications and Specificity?
What if impurity area in control sample coming more as compared to LOQ level of impurity ?
What is the difference between purge septum flow and column flow in gas chromatography?
AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?
why octanol used to determine the partition coefficient ?
we can use expired sample for validation and analyst qualification?
In performance Check of GC Why Hexadecane Peak is ConsideredÂ
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
Why Ethanol is Used for Standardization of GC Head space?
what is the procedure for cleaning of lenses of hatr accesory of ftir instrument?
which are the diffrent batches in the pharmaceuticals?
If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?
i want model written test paper for the post of analyst in IOCL(indian oil corporation ltd) any one pls help me
calibrtion procedue of LC-MS