Answer Posted / ch.h.n.prasad reddy
Peak purity in HPLC is nothing but SPECTRUL PURITY not
Chemical purity. In HPLC Each peak is combination of many
data points. Each data point is having SPECTRUM. In peak
purity calculation each data point SPECTRUM is compared
with SPECTRUM of Peak apex Data point. In this we can
compare deferent region data points with Peak apex Data
point SPECTRUM. At that time we consider Base line noise,
Solvent noise etc.... we can select wave length range
also....
Always Purity angle is less than threshold then only Peak
Purity can pass.
Best Example: Isomers
Both isomers can show same spectrum but chemically they
are different. In this case if we get single peak also our
Peak Purity can Pass because our Peak apex Data point
SPECTRUM and deferent regions in that Peak data points
SPECTRUM is SAME.
Thanks
CH.H.N.PRASAD REDDY
RESEARCH SCIENTIST
prasadreddy19@gmail.com
Is This Answer Correct ? | 104 Yes | 10 No |
Post New Answer View All Answers
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?
How can we calculate "confidence interval" in analytical method validation? Pl. explain with example.
how a particular wavelength can be different for a particular compund while analysing by uv and by HPLC.
what is mean by dissolution biowaiver study?
Why Ethanol is Used for Standardization of GC Head space?
Why only Copper standard is used to calibrate Atomic Absorption spectrophotometer?
i want model written test paper for the post of analyst in IOCL(indian oil corporation ltd) any one pls help me
Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc
what is definition of validation? which components are followed give detail?
if tech transfer fails whay require to do?
1)What's the meaning of Absorption,give a example. 2)What's the meaning of Adsorption,give a example. 2)what is the difference between Absorption and Adsorption.
how to calculate elemental impurities?
what type of question will ask in the interview of lab chemist.
in which situation ion pair require to use?