How to calculate LOD & LOQ in HPLC Validation Method
Answer Posted / balaji
There are three different method indiated in ICH guidelines
for determination of LOD and LOQ.
1 Visual evaution method - For non instrument and sometimes
for instrumental also.
2 Slope Method:Based on residual standard deviation of the
response and
the slope
obtained will be calculated as:
Limit of Detection (LOD):
DL= 3(RESIDUAL SD/Slope)
Limit of quantification(LOQ):
DL=10(RESIDUAL SD/Slope)
3 Signal to noise ratio method
Limit of Detection (LOD):
S/N NLT 3
Limit of quantification(LOQ):
S/N NLT 10
Slope and S/N methods are more reliable
method for determination of LOD and LOQ value
Is This Answer Correct ? | 58 Yes | 11 No |
Post New Answer View All Answers
Why potassium dichromate is used in calibration of uv?
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
Explain the relations between number of carbon atoms in alkanes and retention time ?
why we are using benzene, anyline in acetic anhdride assay titration method?
What is rs test why we are performing rs test?
why multimedia dissolution require to do?
How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?
inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?
what is the different in Total ash, sulphated ash, acid insoluble ash, alcoholic or non alcoholic ash?
what is mean by nitrosamine impurities?
In which situation we require to analytical method validation of excipient?
if content uniformity passing but dissolution varrying then what is next step?
if instrument calibration fails then what require to do?
What is intact assay method development.....?
what is turbidimetric titration?Give 2 examples.