what is method development parameter in HPLC method
development
Answer Posted / vinod kumar, nektar therapeuti
1.If molecular weight less than 2000. based on their
solubility follow as below
2.If water soluble and non ionic follow reverse phase.
3.If water soluble and ionic follow reverse phase ion pair
method.
4.If organic soluble follow normal phase.
5.If molecular weight is more than 2000.Based on their
solubility follow as below.
6.If organic soluble follow gel permeation chromatography
7.If water soluble ion exchange or gel filtration.
8.Once the technique is fixed optimise column.
9.Optimise buffers
10.optimise pH of buffers
11.optimise concentration of buffers.
12.optimise type of organic solvent.
13.optimise column temperature
14.optimise gradient programme to improve resolution and to
elute out late eluting peaks.
Is This Answer Correct ? | 19 Yes | 11 No |
Post New Answer View All Answers
why require to add enzymes in capsules dissolution only? is it require to tablet also?
Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc
What is the accrptance criteria in RSD for RS method precision on basis of impurity percentages?
Which are the diffrent grades of api in pharma?
is it nessesary to do solution stability for 7 days?
in stress study if your api not soluble in hcl naoh h2o2 then what require to do?
How you develop a method in HPLC?
how to develop the icp ms method? Application of icp ms?
In the isomers,enantiomers give the only physical properties present such that no chemical properties present.
What is the acceptance criteria for moisture balance when calibrated with sodium sulfate
some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
if identification threshold crosses the limits then what next step?
How can the GC or HPLC method is selected to determine the impurity profile in drug product?
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
How will u perform degradation study in detectors other thann PDA in HPLC Methodology