what is method development parameter in HPLC method
development
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Answer Posted / yojana
1. Check solubility,if sample dissolved in water then use
reverse phase,othervise use normal phase.
2.wavelength selection
3.selection of mobile phase,buffer.
4. pH OF BUFFER &MOBILE PHASE
5. selection of column
6. check retention time
7 .Flow rate
8. check system suitability parameter like
i)theoritical plates
ii) Assymetry
iii) Resolution
iv) capacitor fator
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