Answer Posted / ramana

Parameters involved during initial stage of Analytical
Method development:

1. Solubility
2. Pka Value
3. Selection of Buffers
4. pH of buffer/mobile phase
5. Composition of Mobile Phase
6. Selection of Column
7. Optimization of Analyte signal
8. Objectives of Seperation
8.1 Analysis time
8.2 Resolution
8.3 k factor (Capacity factor)
8.4 Peak height
8.5 Asyymetry
8.6 Theoritical Plates
9. Flow rate

what is difference in ods I ods II is this is realy from sorbent / packing characteristics or from other means

1956

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Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...

1670

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Why only hydroxy naphthol blue indicator is used for standardisation of 0.05M EDTA solution instead of solochrome Black T or Euriochrome Black T indicator which is used for all sample analysis with 0.05M EDTA solution?

2704

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Difference between the quantitative analysis and qualitative analysis?

1150

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How doing qbd practically?

948

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How to establish relative response factor for hplc. Why it is required?

1201

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We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy

1889

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how you establish the LOQ?

766

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if you have given a blank water how you analyse by general analytical techniques?

2018

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what is the purge flow & how to calculate

2628

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In which situation we require to prepare the standard solution from sample in Related substance method?

762

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What is shaking level in GC?

780

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what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?

4471

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[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?

762

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if instrument calibration fails then what require to do?

967

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