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what is method development parameter in HPLC method
development
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Answer Posted / ramana
Parameters involved during initial stage of Analytical
Method development:
1. Solubility
2. Pka Value
3. Selection of Buffers
4. pH of buffer/mobile phase
5. Composition of Mobile Phase
6. Selection of Column
7. Optimization of Analyte signal
8. Objectives of Seperation
8.1 Analysis time
8.2 Resolution
8.3 k factor (Capacity factor)
8.4 Peak height
8.5 Asyymetry
8.6 Theoritical Plates
9. Flow rate
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