Answer Posted / abdulkalim

Parameters involved during initial stage of Analytical
Method development:

1. Optimization of Mobile Phase
2. Selection of Buffers
3. Selection of Ion pair reagent
4. pH of buffer/mobile phase
5. Composition of Mobile Phase
6. Selection of Column
7. Optimization of Analyte signal
8. Objectives of Seperation
8.1 Analysis time
8.2 Resolution
8.3 k factor (Capacity factor)
8.4 Peak height
8.5 Asyymetry
8.6 Theoritical Plates
9. Flow rate

in OSD forms require to use gas chromatography?

814

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What is Band broading effect explain

1863

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What is the formula for relative diffrence for standard solution in solution stability in validation?

780

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What should be the level/ Percentage of Arsenic in Arsenic free zinc.

2217

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In performance Check of GC Why Hexadecane Peak is Considered 

3023

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what are the standard methods for HPLC?

2776

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2305

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Which is the highly polar and highly non polar column in HPLC?

2285

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in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?

711

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What is the use of tlc and hplc? And when and where use?

1135

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Why we select scan range from higer wavelength to lower wavelength in uv visible spectroscopy and ftir spectroscopy ? .

896

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what is the disso medium for tropsiun chloride

2248

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if instrument calibration fails then what require to do?

967

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How do we fix the sample concentaryion in hplc method development. What is the basis?

1190

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How to calculate coreletion coefficient

1694

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