Interested to Buy Any Domain ? << Click Here >> for more details...
How to calculate LOD & LOQ in HPLC Validation Method
- 14 Answers
- 116157 Views
- Dr Reddys, GSK GlaxoSmithKline, Reddy Labs, Reliance, UIS, I also Faced
- E-Mail Answers
Answer Posted / abdulkalim
Based on standard deviation of the response and the slope
obtained will be calculated as:
Limit of Detection (LOD):
DL= 3(SD/Slope)
Limit of quantification(LOQ):
DL=10(SD/Slope)
| Is This Answer Correct ? | 231 Yes | 20 No |
Post New Answer View All Answers
how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,
What is Band broading effect explain
Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?
What is control room temperature and which guide line says?
Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?
what type of question will ask in the interview of lab chemist.
calibrtion procedue of LC-MS
How will u perform degradation study in detectors other thann PDA in HPLC Methodology
iam usig ph buffers merk. manually how to prepare ?
why we are using hexane in calibration of number of drop per mL
from where i get mortar pastle for glass bottle crush? it's required for testing of it?
what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?
I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
What is related substance by HPLC impurity limits as per USP?
