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why we wre doing linearity siudiec in hplc method validation

Answer Posted / k.praveen kumar macleods pharm

If the method is linear or not from certain range suppose
in assay validation the range is 50% to 150% we are
injecting minimum 6 levels and also Calculate the regration
line the correlation coefficient should be less than 0.999
Here regration line equation is y=mx+c

where y = Regration line
m = Slope
c = Intercept.

and also calculate residual sum of squares from theorical
and practical

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