Answer Posted / vinee
Esculin hydrolysis is recommended in the differentiation and
identification of a variety of organisms. If the test
organism does not hydrolyze esculin, the medium remains
unchanged and the esculin will fluoresce when subjected to
long-wave UV light at 360 nm. When hydrolyzed, the medium
turns black and fluorescence is lost.
Esculin is a glycoside incorporated as a differential agent
to facilitate the identification of various organisms,
including Enterobacteriaceae, enterococci and anaerobes.
Hydrolysis of esculin yields esculetin and dextrose. In the
presence of an iron salt, esculetin forms a brown-black
complex that diffuses into the surrounding medium
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