what is the difference of 293T, HEK293 and HEK293T?
Answer Posted / andrew
They are all Human Embryonic Kidney cells (well they are supposed to be, but there is some dispute over their actual cell type with some suggestions they may be neuronal). HEK293 are the original cell line derived and are used for the easy transfectability. HEK293T cells have been transduced with the SV40 (virus) large T antigen, which permits the amplification of plasmids containing the SV40 origin of replication. Thus even if only one plasmid makes it into these cells, many copies can be made permitting both higher levels of expression and longer retention of the plasmid following multiple rounds of mitosis.
Is This Answer Correct ? | 11 Yes | 0 No |
Post New Answer View All Answers
What are heterochromatin and euchromatin?
how to detect and de-contaminate, Mycoplasma contamination?
what is the role of L-glutamine in RPMI?
How C2C12 cells model for differentiation?
Does calcium protect cells from chemotrypsin ?
Explain how many cells are in the average human brain?
How does the sodium-potassium pump present in the cell membrane work? What is the importance of this protein for the cell?
Describe about equine fibroblast culture?
Explain about the plating efficiency for transfection ?
What are homologous chromosomes? Which are the human cells that do not have homologous chromosomes?
Describe cell membrane permeability ?
What do you know about THP-1 cell line ?
What are chloroplasts? What is the main function of chloroplasts?
How to Transport mouse splenocytes ?
Describe the procedure for isolating endothelial cells from mouse lung?