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what are the five greatest discoveries in the field of r DNA
technology.

Answer Posted / anuj kumar pandey

1. restriction endonucleases.
2. ligases
3. taq DNA polymerase & other thermostable polymerase.
4.PCR
5. MODIFYING ENZYMES e.g: alkaline phosphatase & polynucleotide kinase.
6.techniques used in construction & screeing of cDNA & genomic libraries.
7. Techniques to investigate the expression of genes at the levelof transcription & translation level.

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