Answer Posted / rishu
Enables proteins and peptides to be sequenced.
Pehr Edman introduced phenylisothiocyanate (Edman's
reagent) to selectively label the amino-terminal residue of
a peptide. In contrast to Sanger's reagent, the
phenylthiohydantoin (PTH) derivative can be removed under
mild conditions to generate a new amino terminal residue
(Figure 4–7). Successive rounds of derivatization with
Edman's reagent can therefore be used to sequence many
residues of a single sample of peptide. While the first 20–
30 residues of a peptide can readily be determined by the
Edman method, most polypeptides contain several hundred
amino acids. Consequently, most polypeptides must first be
cleaved into smaller peptides prior to Edman sequencing.
Is This Answer Correct ? | 15 Yes | 2 No |
Post New Answer View All Answers
What are the favorable conditions for formation of Anions?
How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?
What does thermodynamics helps in predicting?
Give the chemical formula for Borax?
What is polymerase chain reaction used for?
What is the enzyme that catalyzes the production of rna?
What is the bond angle of phosphorous?
What are allosteric enzymes?
Aromatic compounds generally undergo which type of reactions?
What is the tertiary structure of a protein?
What are hydrophobicity scales, and how are they used?
What are respectively some remarkable functions of cd4?
Is there any situation in which dna is made based on a rna template? What is the enzyme involved?
How can we calculate the number of possible optical isomers for a given compound?
Differentiate between gram +ve and gram -ve bacteria.