Answer Posted / swapna
Step 1. Assemble your sequences.
Put all your sequences in Fasta format in a single file.
This file should be located in a suitably named
subdirectory of your home directory on the UBiC Blast
server. The definition line for each sequence should start
with a unique identifier for that sequence.
Step 2. Convert this sequence file to a Blastable database.
The command formatdb converts your Fasta file of sequences
to a set of files that can be queried with command-line
BLAST.
Step 3. Test Blast on your database.
See the UBiC tutorial, Using Command-line BLAST. In the
blastall command-line you will need to specify the location
of your database by typing: -
d /disk2/home/myhome/blastdbs/custom.aa.
Is This Answer Correct ? | 1 Yes | 0 No |
Post New Answer View All Answers
What is the secondary structure of intron ?
How do you find the most recent human sequence data in the data bases inorder to determine physical maps?
How to find a sequence of dna molecule?
Are all members of transporter families confined to a particular organelle or to the plasma membrane, or are some family members found throughout cellular compartments in eukaryotes?
Can independent origins be established for two families of transport systems having no sequence or motif similarities without three-dimensional structural data?
What are the current challenges in the Pharmaceutical and biotechnology sectors?
What is a homologue?
How to find coexpressed genes on NCBI GEO?
Does multidrug resistance (mdr) arise by activation of stable genes encoding drug efflux pumps or by mutations of genes encoding other types of transporters in bacterial pathogens?
What is the criteria for LTQ (LC-MS/MS) SEQUEST search?
What is C-compliator?
Did integral membrane transport proteins arise as an independent protein class or from other types of proteins?
Integrate 3x + 5 / (x3-x2-x+1)?
What is protein sequencing?
What is enzyme-ligand interaction?