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Answer Posted / vinee
DNA shuffling(Sexual PCR), in contrast, is PCR without
synthetic primers.
In this process, a family of related genes--say, the ones
that codes for the surface proteins of three different HIV
isolates--are first chopped up with enzymes.
The gene fragments then are heated up to separate them into
single-stranded templates. Some of these fragments will
bind to other fragments that share complementary DNA
regions, which in some cases will be from other family
members.
Regions of DNA that are non-complementary hang over the
ends of the templates (see illustration). The PCR reaction
then treats the complementary regions as primers and builds
the new double-helical DNA.
But PCR also adds bases to the overhanging piece of the
primer, forming a double helix there, too. This ultimately
creates a mixed structure or chimera. In the final step,
PCR reassembles these chimeras into full-length, shuffled
genes.
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