Answer Posted / Divya Saurabh
ELISA (Enzyme-Linked Immunosorbent Assay) is a versatile immunoassay technique used to quantify or detect substances (antigens, antibodies, hormones, etc.) in a sample. The basic principles involve coating a solid surface with the antigen, adding a solution containing the substance of interest, washing away unbound substances, introducing an enzyme-labeled antibody specific to the substance, and finally adding a substrate that reacts with the enzyme to produce a measurable signal. The intensity of the signal corresponds to the amount of the substance in the original sample.
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