How to reduce cell clumping for immunocytostaining ?
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Answer Posted / Shashibala
To minimize cell clumping during immunocytostaining, it is essential to maintain a low cell density in the culture and use a gentle trypsinization method. Additionally, using a narrow-bore pipette tip can help to disperse cells and reduce clumping. Centrifugation at low speed and resuspending the pellet gently in an appropriate volume of medium can also aid in reducing cell clumping.
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