give clarity of linearity and range
in method validation
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Answer Posted / Ram Saheli
In analytical chemistry, linearity is a measure of the relationship between the concentration of an analyte (the substance being measured) and its response (such as absorbance or mass). A calibration curve is used to determine this relationship, with known concentrations of the analyte plotted against their responses. The slope and intercept of the best-fit line can then be used to calculate the concentration of unknown samples.nnRange refers to the range over which a method is considered valid for quantitative analysis. This is typically determined by performing a calibration curve with a series of known concentrations that cover the expected range of analyte concentrations in the sample.nnIn method validation, it is important to verify linearity and range to ensure accurate and reliable results.
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