In HPLC Chromatographic separation Resolution equation is
Rs = 1.18 (tR2 –tR1)/wh1+Wh2,
Where, tR2 and tR1 is run time of eluent ratios of the
distances from the point of application to the centers of
the spots and the distance travelled by the solvent front
from the point of application (retardation factor), and
Wh1and Wh2 , is peak width of at half height.
What is 1.18 ?
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Answer Posted / bhavin
In a peak with Gaussian distribution, the peak width is W = 4 σ (where σ is the standard deviation) and the peak FWHM is W0.5h = 2.354σ. hence 1/2*2.354=1.177=1.18
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