water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.
- 10 Answers
- 48802 Views
- Matrix, I also Faced
- E-Mail Answers
Answer Posted / prashant
It based on affinity of eluting substance with stationary phase/mobile phase.if compound elute earlier when we increase ACN obviously compound has more affinity with ACN (towards nonpolar) so it breaks wonderwal bonds with stationary phase and elute earlier
| Is This Answer Correct ? | 2 Yes | 0 No |
Post New Answer View All Answers
A ha tc is actual defination of retention time?
why should we perform dissolution PVT calibration only by UV spectrometer, not by HPLC ?
in each media we require to use SLS? how to proceed?
what is third integration
While performing TOC sst analysis Zero shift disabled & sample analysis zero shift enabled why?
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
How to calculate coreletion coefficient
What is similarity factor?
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?
which are the diffrent batches in the pharmaceuticals?
why octanol used to determine the partition coefficient ?
why we use glass fiber filters use in some situation?
How can I develope method of dissolution by HPLC OR UV
What is control room temperature and which guide line says?
