What is the principle of GC/HPLC ?
Answer Posted / manigandan p
This both are separation technique.
GC Principle: Sample vapourised by injection in to a heated system, eluted through column by inert gaseous mobile phase and detect.
HPLC Principle: The solvent liquid will pressurize the sample through column. Column filled with adsorbent material. Each components in the sample interacts slightly differently with adsorbent material. It causes different flow rates for different components and its leading to separate.
Is This Answer Correct ? | 20 Yes | 0 No |
Post New Answer View All Answers
How do we fix the sample concentaryion in hplc method development. What is the basis?
Why we use potassium dichromate in uv calibration Exact reason behind it??
How to do regeneration of Metacarb Pb plus column?
A ha tc is actual defination of retention time?
when we talk about change in entropy we consider total entropy means of surround +entropy of system but when we talk about change in Gibss free energy we consider entropy of system.explain with reason.
what is mean by dissolution hydrodynamics?
what is the principle of UV Vis spectroscopy, AAS, ICP OES,ICPAES, ICP-MS and FTIR
which are the sizes of capsules?
in which situation ion pair agent require to use?
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
how you confirm the assay method?
in stress study if your api not soluble in hcl naoh h2o2 then what require to do?
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
how to calibrate hplc & gc
if you have given one product then how you determine the impurity in that?