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What is the principle of GC/HPLC ?
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Answer Posted / manigandan p
This both are separation technique.
GC Principle: Sample vapourised by injection in to a heated system, eluted through column by inert gaseous mobile phase and detect.
HPLC Principle: The solvent liquid will pressurize the sample through column. Column filled with adsorbent material. Each components in the sample interacts slightly differently with adsorbent material. It causes different flow rates for different components and its leading to separate.
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