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What are the limitations of blotting techniques and what
alternatives can you suggest?
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Answer Posted / abe
Blotting techniques show many limitations in comparison to other molecular techniques. It depends what you're attempting to look for, it's very situational.
- They take up alot of time
- They degrade protein tertiary structure
- They show limited quantitative abilities
- They show lower specificity and sensitivity compared to other techniques.
If you're looking for gene expression I would say reverse-transcriptase PCR is a more appropriate alternative.
Here is a good article discussing these limitations:
http://www.nature.com/jid/journal/v133/n7/pdf/jid2013216a.pdf?wt.ec_id=jid-201307
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