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Answer Posted / rambabu cherukuri
Endcapping is a chemical procedure to reduce the polar nature of silica in RP HPLC coloumns by attaching the trimethyl silane to free silanol group
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Why to use propyl paraben in HPLC gradient calibration
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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