Answer Posted / bem nyax

This method exploits the property that alkali halides become plastic when subjected to pressure and form a sheet that is transparent in the infrared region. Potassium bromide (KBr) is the commonest alkali halide used in the pellets. Cesium iodide (CsI) may also be used to measure the infrared spectrum in the 400 to 250 cm-1 low-wavenumber region. 
The preparation method for 13 mm-diameter pellets is described below. Approximately 0.1 to 1.0 % sample is well mixed into 200 to 250 mg fine alkali halide (the example of KBr is used below) powder and then finely pulverized and put into a pellet-forming die. A force of approximately 8 tons is applied under a vacuum of several mm Hg for several minutes form transparent pellets. Degassing is performed to eliminate air and moisture from the KBr powder. Inadequate vacuum may result in easily broken pellets that scatter light. Before forming the KBr powder into pellets, pulverize it to 200 mesh max. and then dry at approximately 110 °C for two to three hours. Note that rapid heating can oxidize some of the KBr powder to KBrO3, causing a brown discoloration. After drying the powder, store it in a desiccator. 
When performing measurements, the background can be measured with an empty pellet holder inserted into the sample chamber. However, background measurements on a pellet holder with a pellet of KBr only, that contains no sample, can correct for infrared light scattering losses in the pellet and for moisture adsorbed on the KBr.

Reference >> http://www.shimadzu.com/an/ftir/support/ftirtalk/talk8/intro.html

Which parameter require to do for analytical method equivalency?

774

---

what are the standard methods for HPLC?

2786

---

1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer

1848

---

How do we fix the sample concentaryion in hplc method development?

1136

---

which are the diffrent batches in the pharmaceuticals?

816

---

In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.

3127

---

how a particular wavelength can be different for a particular compund while analysing by uv and by HPLC.

2287

---

What is delay volume?

1391

---

if you have given one product then how you determine the impurity in that?

738

---

What is intact assay method development.....?

2661

---

Please tell me about the pH of Polycaboxylic ether is it in the 5-6 range ever or more than 6

2609

---

if instrument calibration fails then what require to do?

978

---

How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?

1776

---

We use hicrome 10 cxs column in hplc analysis....pls tell me.. what is the meaning of 10 cxs?

1717

---

WHICH STANDARD WE USE IN SILICA ANALYSIS IN SILICA-GEL METHOD FOR SOLID SAMPLE

2132

---