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Answer Posted / manoj kumar
Ans no first is correct. And very clear and effective for a chemist as well as student.
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In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
all types of questions asked at the cipla interview
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how to select short coloum and long coloumn for new molecule
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why require the ph, buffer during hplc mobile phase?
