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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / madhan rahul
Caffeine is the universal primary standard and it is recamanded by pharmacopiea.
Caffeine having variable wavelengths maximum at 204 ,273 and minimum is 245 nm.
It is freely soluble in water and also stable in room temperature.
Its highly polar and elutes all C 18 columns at Retention time about 2 minutes.
In pharma maximum products are using 200 to 300 nm only so caffeine is used for detector calibration.
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