When i inject amines and acid on HPLC then which peak will
elut first?
- 4 Answers
- 11743 Views
- Sun Pharma, I also Faced
- E-Mail Answers
Answer Posted / dhramveer
Elution of amine and Acid depends upon pH of mobile phase and pKa of molecule. If the mobile phase pH is acidic then Amine will elute first but if the pH is alkaline >7 , then acid may elute first.
| Is This Answer Correct ? | 7 Yes | 1 No |
Post New Answer View All Answers
If vendor having more imp than monograph then how to proceed? and how to set spec?
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
is it necessary to do HPLC calibration at wavelength 315nm if we are doing analysis at this wavelangth
What is the Formula for coreletion coefficient in plhplc calibration
identification is for unknown? qualification for known? reporting for LOQ?
why digestion require in icpms?
what type of questions asked on analytical balance and also give answers................all pharma companies?
on the basis of bcs class how to proceed the solubility?
In IR spectra, generally a molecule vibrates without apply emr or ir light?
what is partion and column chromatography
How we can identify process related and degradation impurity in single method with short period?
how much mass should be there in volumetric flask while in preparation of sample for assay?
Why we use potassium dichromate in uv calibration Exact reason behind it??
in hplc chromatogram started from left to right and in uv spectrum started from left to right
why octanol used to determine the partition coefficient ?
