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What is the Baseline in HPLC.

Answer Posted / kanhu charana sahu

In chromatography, the base line is the name given to that part of a chromatogram that represents any time period during which only mobile phase is passing through the detector. Any signal present on the base line represents the noise of the chromatographic system. In quantitative analysis, the base line represents the reference point for integration when measuring the area of any given peak.

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