what is difference of uv and pda
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Answer Posted / rajib hazarika
1. By PDA detector, we can measure the area/height
of particular sample at wavelengths ranging from
190 to 800 nm by a single injection( 3D scan is possible(Response Vs time Vs wavelength). Whereas in uv detector scanning is not possible.)
2. for unknown sample with unknown lambda maximum with a single injection PDA we can determine the lambda maximum but by UV several injection is required with different wavelength.
(for method validation, process validation, Research and development PDA is prefered as compared to UV
3. If proper elution is established with the help of multi step gradient mobile phase and column then analysis of mixture of compounds(with different Lambda max) can be possible by a single injection in PDA.
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