Interested to Buy Any Domain ? << Click Here >> for more details...
Give the comparisons of Rec-type (proteine) and Rec BCD
Complex. What is the role of each?
- 1 Answers
- 6039 Views
- National University of Rwanda, I also Faced
- E-Mail Answers
Answer Posted / ntegerejimana theogene
RecBCD (EC 3.1.11.5, Exonuclease V, Escherichia coli
exonuclease V, E. coli exonuclease V, gene recBC endoenzyme,
RecBC deoxyribonuclease, gene recBC DNase, gene recBCD
enzymes) is an enzyme of the E. coli bacterium that
initiates recombinational repair from potentially lethal
double strand breaks in DNA which may result from ionizing
radiation, replication errors, endonucleases, oxidative
damage, and a host of other factors.[2][3] The RecBCD enzyme
is both a helicase that unwinds, or separates the strands of
DNA, and a nuclease that makes single-stranded nicks in DNA.[1]
Both the RecD and RecB subunits are helicases, i.e.,
energy-dependent molecular motors that unwind DNA (or RNA in
the case of other proteins). The RecB subunit in addition
has a nuclease function.[5] Finally, RecBCD enzyme (perhaps
the RecC subunit) recognizes a specific sequence in DNA,
5'-GCTGGTGG-3', known as Chi (sometimes designated with the
Greek letter χ).
RecBCD is unusual amongst helicases because it has two
helicases that travel with different rates[6] and because it
can recognize and be altered by the Chi DNA sequence.[7][8]
RecBCD avidly binds an end of linear double-stranded (ds)
DNA. The RecD helicase travels on the strand with a 5' end
at which the enzyme initiates unwinding, and RecB on the
strand with a 3' end. RecB is slower than RecD, so that a
single-stranded (ss) DNA loop accumulates ahead of RecB
(Figure 2). This produces DNA structures with two ss tails
(a shorter 3’ ended tail and a longer 5’ ended tail) and one
ss loop (on the 3' ended strand) observed by electron
microscopy.[9] The ss tails can anneal to produce a second
ss loop complementary to the first one; such twin-loop
structures were initially referred to as “rabbit ears.”
Notes: If you are not satisfied , contact me. these are my
cell phones: +250784944242 or (+250)728944242. or write for
me on my this E-mail: ntegerejimanat@gmail.com
| Is This Answer Correct ? | 2 Yes | 0 No |
Post New Answer View All Answers
What do you mean by bio-media?
in the lcmv what type of effect onm the mhc class i molecule
What are the different types of bio technology? Explain.
How to Filter & remove non-sequence data from a FASTA FILE using Perl scripts ?
what are some of the concerns about biotechnology regarding human health and biodiversity?
Hi i'm going to attend online test conducted by BCIL in few days...pls anyone can tell me about the test and how to prepare for it...
describe the cell theory & discuss the parameters for cell differentiation
Does eukaryotes have restriction modification system?
Why do you use dbm instead of ncf in the northern blotting and what are the pre-treatments required for this paper to be used?
Hai friends, Can someone guide me..Am a postgraduate(m.tech) in biotechnology and am thinking of doing pg diploma course through distance mode. are they worth doing? if so which is better pg diploma in bioinfo or pg diploma in Intellectual property rights..am awaiting ur suggestions
What are the problems we could face by using this genetic engineering tools?
how do you get on with your current boss
What do you understand by the term “Bio-Technology”?
Give an example for electrophilic substitution reaction?
How to isolate prokaryotic mRNA
