Interested to Buy Any Domain ? << Click Here >> for more details...
Give the comparisons of Rec-type (proteine) and Rec BCD
Complex. What is the role of each?
- 1 Answers
- 6125 Views
- National University of Rwanda, I also Faced
- E-Mail Answers
Answer Posted / ntegerejimana theogene
RecBCD (EC 3.1.11.5, Exonuclease V, Escherichia coli
exonuclease V, E. coli exonuclease V, gene recBC endoenzyme,
RecBC deoxyribonuclease, gene recBC DNase, gene recBCD
enzymes) is an enzyme of the E. coli bacterium that
initiates recombinational repair from potentially lethal
double strand breaks in DNA which may result from ionizing
radiation, replication errors, endonucleases, oxidative
damage, and a host of other factors.[2][3] The RecBCD enzyme
is both a helicase that unwinds, or separates the strands of
DNA, and a nuclease that makes single-stranded nicks in DNA.[1]
Both the RecD and RecB subunits are helicases, i.e.,
energy-dependent molecular motors that unwind DNA (or RNA in
the case of other proteins). The RecB subunit in addition
has a nuclease function.[5] Finally, RecBCD enzyme (perhaps
the RecC subunit) recognizes a specific sequence in DNA,
5'-GCTGGTGG-3', known as Chi (sometimes designated with the
Greek letter χ).
RecBCD is unusual amongst helicases because it has two
helicases that travel with different rates[6] and because it
can recognize and be altered by the Chi DNA sequence.[7][8]
RecBCD avidly binds an end of linear double-stranded (ds)
DNA. The RecD helicase travels on the strand with a 5' end
at which the enzyme initiates unwinding, and RecB on the
strand with a 3' end. RecB is slower than RecD, so that a
single-stranded (ss) DNA loop accumulates ahead of RecB
(Figure 2). This produces DNA structures with two ss tails
(a shorter 3’ ended tail and a longer 5’ ended tail) and one
ss loop (on the 3' ended strand) observed by electron
microscopy.[9] The ss tails can anneal to produce a second
ss loop complementary to the first one; such twin-loop
structures were initially referred to as “rabbit ears.”
Notes: If you are not satisfied , contact me. these are my
cell phones: +250784944242 or (+250)728944242. or write for
me on my this E-mail: ntegerejimanat@gmail.com
| Is This Answer Correct ? | 2 Yes | 0 No |
Post New Answer View All Answers
why the word trasfection is prefers for transfer of genetic material through non viral particles ineukaryote not for prokaryote?
How is this bio technology useful in our day to day life?
I have an elder brother who is married and settled in USA.Around 2 1/2years back even he had gone for his masters. I would be appearing for Visa soon. I have got acceptance fron Wayne state Univ for masters in biotechnology.What should my answer be when this question is raised. Would my case become more critical? please help
What do you understand by the term “Bio-Technology”?
What is the difference between defined, characterized and standard serum?
How would you value a biotech company as opposed to a consumer products company?
if u've any queries or need more info about biocon interviews. u jus mail to raaju_kaarthikeyan@yahoo.co.in
How to Filter & remove non-sequence data from a FASTA FILE using Perl scripts ?
What are the different types of bio technology? Explain.
will u pls suggest me which books should i prepare for group 2. and where will i get that books. pls pls
What topics cover the study of biomedical sciences?
what are the conditions and time requirements for carrying out the various steps involved in northern blotting?
What is the definition of 'biomedical'?
How to Calculate Carbohydrate percentage in dubois method?
Why do you use dbm instead of ncf in the northern blotting and what are the pre-treatments required for this paper to be used?
