Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / satish setti
1.caffine having mulltiple wavelengths 205,254,273nm.so we can use it at multiple wavelengths.
2.it is primary standard so stable un nature.
3.availability and freely soluble in methanol.
Because of above reasons caffine can be used in calibration.
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