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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / satish setti
1.caffine having mulltiple wavelengths 205,254,273nm.so we can use it at multiple wavelengths.
2.it is primary standard so stable un nature.
3.availability and freely soluble in methanol.
Because of above reasons caffine can be used in calibration.
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In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
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