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Why caffeine as a reference standard used for calibration
of HPLC?
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Answer Posted / neelam tyagi
it shows maxima at the multi wavelength of 205 nm and 273
nm shows and shows minima at the wavelength of 245
nm. It is a Primary reference standard andvery easily
avaliable
in the market .It is cheap.It is non-degradable
and we can used it for longer period of analysis it is very
highly stable
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