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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / madhu
i said one my interview it is low cost,multiple wave lengths but these are not correct he said.any person know exact reason tell me.
but i think in uv spectroscopy some electronic bonding,non bonding transitions by c-n,c=c,c=n,n-o interactions.then it gives different wavelenghths with in the uv region only other compounds its not easily detectable
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