Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
- 187994 Views
- Arvind, Aurobindo, Cipla, Reddy Labs, Shasun Chemicals, I also Faced
- E-Mail Answers
Answer Posted / srinivas
caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.
It is very stable, we can use a long time foranalysis
i.e. it is not degradable
| Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
How to calculation of residual solvent methanol (3000 ppm) standard solution, and how many quantity used of test sample
What is third generation HPLC Columns?
why octanol used to determine the partition coefficient ?
WHAT IS THE CRITERIA FOR SELECTION OF TIME OF DISSOLUTION AND THE MEDIUM OF DISSOLUTION?
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?
what is %labelled amount in content uniformity of dosage unit and its calculation?
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
why digestion require in icpms?
What is viod volume and peak purity in HPLC?
what is third integration
what is mean by ambient temperature?
In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )
what is mean by extactable and leachable study?
What are modes of quantification in related substances
