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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / srinivas
caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.
It is very stable, we can use a long time foranalysis
i.e. it is not degradable
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