When i inject amines and acid on HPLC then which peak will
elut first?
Answer Posted / manjeera
Elution of the compound depends on the type of chromatography whether it is reverse phase or normal phase chromatography.in reverse phase mobile phase will be polar so polar compound elutes first and vice versa for normal phase chromatography.
Is This Answer Correct ? | 29 Yes | 1 No |
Post New Answer View All Answers
Why dissolution test is not performed in all of the products
if your impurity coeluting with each other in that situation how require to set specification? is it acceptable?
which one is better separation in high pressure liquide chromatogram orlowpressure chromato gram in hplc
how require to select dissolution media? what is discrimination?
in which situation ion pair agent require to use?
How require to perform linearity as per ANVISA? What are the acceptance criteria?
what is mean by nitrosamine impurities?
What is similarity factor?
WHAT IS THE USE OF STARTER ?????????
in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?
what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?
What is the formula to determine the concentration of M of a solution given the % transmittance? Use %T = 43.7 as an example.
How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?
Difference between the quantitative analysis and qualitative analysis?
what is diffrence between specificity and selecivity?