Interested to Buy Any Domain ? << Click Here >> for more details...
water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.
- 10 Answers
- 52707 Views
- Matrix, I also Faced
- E-Mail Answers
Answer Posted / paresh jobanputtra, piramal(ah
Actually What happen,When we mix Acetonitrile with buffer then VISCOSITY is decresed of mobile phase.
"As viscosity decreased, Mass transfer of solute particle with stationary phase is decreased"
So but obvious component elute earlier.
Regards,
Paresh Jobanputtra
| Is This Answer Correct ? | 7 Yes | 3 No |
Post New Answer View All Answers
what is third integration
I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
How to know the estimated LOQ concentration in ppm
why lactose has less charges when packed in steel containers when compared with packing of lactose in polyamide material?
What is a difference between potency and purity?
what is mean by covalidation
What is column in chromatography?
give clarity of linearity and range in method validation
Why only hydroxy naphthol blue indicator is used for standardisation of 0.05M EDTA solution instead of solochrome Black T or Euriochrome Black T indicator which is used for all sample analysis with 0.05M EDTA solution?
Home heating oil or furnace oil what is made from? Can (base oil plus diesel/kerosen) = home heating oil?
In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )
what is mean by extactable and leachable study?
4. Describe the operation of the Craig apparatus. Chapter.3 Equilibrium Processes in Separations
Why six unit used for precision?
what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?
