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Why caffeine as a reference standard used for calibration
of HPLC?
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Answer Posted / kalpesh
# it is used for wavelength accuracy for HPLC
# It gives a multiwavelength response like 205,245,273 nm
# 205nm maxima, 245nm minima, 273nm maxima
# It is easily available in market
# It is very stable, we can use a long time for analysis
i.e. it is not degradable
# It is chip
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