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Is it possible to express the genes of prokariots into
eukariots?
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Answer Posted / priyanka
Yes. Both eukaryotes and prokaryotes use the same system of
codons (they speak the same 'language'). For example, GGG
will be translated to glycine in a human and in E. coli
bacteria. Therefore, as long as the eukaryotic gene is
attached to the appropriate prokaryotic gene regulation
elements, a prokaryotic cell will be able to translate and
express the gene. The most common method to accomplish this
is to put the eukaryotic gene in a 'vector' (most commonly a
plasmid of bacterial origin), and then transform the
bacteria with this vector.
This is how humulin (human insulin) was first prepared by
recombinant DNA technology. The eukaryotic genetic
information (human DNA) was inserted into prokaryotic
plasmids, and the bacteria then synthesized insulin.
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