water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.
Answer Posted / muralikrishna
Even though water is a polar sovent the elution strength of
Acetonitrile is more than water.
| Is This Answer Correct ? | 52 Yes | 12 No |
Post New Answer View All Answers
How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?
if rsd failed then what require to do?
why xterra column require to use at higher ph?
Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?
why octanol used to determine the partition coefficient ?
Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?
What is the diffrence in japan mkt requirement in analytical method validation over US?
In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.
in which situation require to change rs specification?
A ha tc is actual defination of retention time?
What is a difference between potency and purity?
in sampling of raw material which side is pasted sampled labels
What is the principle of Thermal conductivity detector and FID?
Why to use propyl paraben in HPLC gradient calibration
In the HPLC Calibration done wavelength accuracy done between 200nm-280nm .but not done remaining 300-400nm not done ?