IF THE COMPOUND PKA IS 5.3 WHY WE ARE ADJUSTING PH+/-2 OF
THE COMPOUNDS PKA. WHAT IS THE CONDITION
Answer Posted / ramya sarma
pKa=pH+log [un ionised]/[ionised]
at the half neutralisation point
un ionised concentration= ionised concentration
so pKa=pH+log1
pKa=pH because log1=0
if we didnt adjudt pH +/-2.0 we may get peak split because
for un ionised we get one peak and ionised we get one more
peak
Is This Answer Correct ? | 1 Yes | 2 No |
Post New Answer View All Answers
What is third generation HPLC Columns?
How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?
What is the principle of Thermal conductivity detector and FID?
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio
What if impurity area in control sample coming more as compared to LOQ level of impurity ?
4. Describe the operation of the Craig apparatus. Chapter.3 Equilibrium Processes in Separations
in sampling of raw material which side is pasted sampled labels
what is biorelivent dissolution media?
how to qualify the impurity?
in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?
Identify problem faced when mass spectroscopy is used with HPLC system ?
What is control room temperature and which guide line says?
if you get peak in blank then what require to do?
What is split ratio in Gc? Splitless? how requirr to select?