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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / sreehari kumar annamraju
caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.It is easily soluble in water. It shows
complete absorbance at multiwavelength,caffeine easily available in market.
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