Answer Posted / balaji

first check the structure of the compound.identify the
chromophore groups(for UV Detector),refractive index status
(for RI Detector),reactive status with other chromophore
group compounds(Derivitation method for UV.then check the
solubility status of the compound.prepare a known
concentration solution.

In HPLC select a C18 Column which can serve better for the
polar and not polar compounds.select a buffer pH Oof +/-1
unit of pka value of the compound prepare a buffer mix with
some 20% of organic solvent to chnge the elution early
(based on polarity)

inject the prepared known concentration sample solution in
to above system by observing the chromatogram procced
further by altering buffer
pH,COLUMN,FLOW,TEMPERATURE,ORGANIC RATIO Sample size etc.

but to quantify the unknown concentration we need a known
concentration of standard.



2304

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Which are the diffrent grades of api in pharma?

743

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What is similarity factor?

1184

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How to calculate coreletion coefficient

1691

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WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?

1169

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if you given one product then which tests you will perform?

692

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If combination product how require to identify which imp is of which api?

823

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can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?

2168

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mode of absorption in alimentary canal?

2371

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CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?

1188

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in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?

709

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How much is the standard area for glc analysis

2261

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Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc

1452

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what is the extinction coefficient for Indomethacin or at 319nm wave length.

4048

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what is mean by covalidation

761

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