Interested to Buy Any Domain ? << Click Here >> for more details...
why we use caffeine for hplc calibration?
- 5 Answers
- 28200 Views
- Aurobindo, I also Faced
- E-Mail Answers
Answer Posted / bhagavanji nelakanti
Caffeine is a primary standard which is having good
qualities like very soluble in methanol, highly stable at
room temperature and given set of conditions also. It is
having high boiling point, cheap in cost, easily available.
It is easy to prepare and handle and give good responses
(lambda max) at 205nm and 272 nm, (lambda Min) at 244 nm. We
can identify the difference in response with changing of
single nm also(271-273nm).
| Is This Answer Correct ? | 50 Yes | 0 No |
Post New Answer View All Answers
how require to interprit the method precision data of hptlc?
Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?
How to do regeneration of Metacarb Pb plus column?
why xterra column require to use at higher ph?
If vendor having more imp than monograph then how to proceed? and how to set spec?
how to select short coloum and long coloumn for new molecule
how we can identify the impurity is coming below loq at transfering site?
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
What is the difference between chromatographic purity and related substances?
Related substance method equivalency on control sample or spiked sample?
what is mean by peak intigrity?
what is difference in ods I ods II is this is realy from sorbent / packing characteristics or from other means
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.
While performing TOC sst analysis Zero shift disabled & sample analysis zero shift enabled why?
