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Answer Posted / ch. abdul hameed kashmiri
1- take 10 clean test tubes
2-take 1mL of serum in 1st tube
3-3-take .5mL of N.S in the remaining tubes
4-take .5mL from tube 1 and shift to the next upto 9th and discard from 9th tube 0.5ml
4incubate the tubes in rack in incubator at 37C for 30 mints
5-centrifuge the tubes at 2000 rpm for 3mints and observe agglutination
6-record the results by comparing it to the control
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