Interested to Buy Any Domain ? << Click Here >> for more details...
what is the method used to identify your transformed cells
from the plasmid vector?
- 2 Answers
- 7657 Views
- I also Faced
- E-Mail Answers
Answer Posted / kk pandey
I)If transformant is thought to have antibiotic resistant
gene (called as Marker Gene)along with ur desired gene, then
culture those cells in a media containing that same
antibiotic.
==>Case 1:- the cell is not transformed.:-
the cell will not have the antibiotic resistant gene, hence
it wont grow (or simply die)bcoz of antibiotic in the media.
==>Case 2:-the cell is successfully transformed.:-
The cell will easily grow in media bcoz antibiotic wont
affect it as cell will resist it.(bcoz of marker gene).
-----*----
My answer is quite long but i think i will properly make u
understand.
| Is This Answer Correct ? | 8 Yes | 2 No |
Post New Answer View All Answers
Is biotechnology more dangerous to other gene transfer technologies breeding?
To how much extent skin color depends on genes?
How will you differentiate alleles of a gene in the double strand of DNA?
cancer cells have their dna wound back wards, is that true?
What are the dangers of Genetically Engineered foods?
What is the chemical composition of gene?
What ethical dilemmas should be considered when genetic engineering is put into practice?
what is Avian flu threat? what are its symptoms?
In hogs a gene that produces a white belt around the animal's body is dominant over its allele for a uniform body. Another independent dominant gene produces fusion of the hoof (syndactyly). Suppose a uniformly colored hog homozygous for syndactyly is mated with a normal footed hog homozygous for the belted character. What would be the phenotype of the F1? If the F1's interbreed, what would be the phenotypic ratio of the F2?
Is Genetic engineering the best of solution?
Flower position, stem length, and seed shape were three traits that were studied by Mendel. Each is controlled by an independently assorting gene. If a plant that is heterozygous for all three traits was allowed to self- fertilize, what proportion of the offspring would be expected to show all three dominant phenotypes?
In mice colored coat is dominant to albinism. Sniffles, a male mouse with a colored coat is mated to Esmeralda, an alluring albino. The resulting litter of six young all had colored fur. The next time around Esmeralda was mated with Whiskers, who was the same color as Sniffles; some of this litter were white. a)What are the probable genotypes of Sniffles, Esmeralda, and Whiskers? b) If a male of the 1st litterwas mated to a colored female of the 2nd litter, what phenotypic ratio might be expected among the offspring? c)What would be the expected results if a male of the 1st litter was mated to an albino from the 2nd?
Does postraumatic stress mutate genes?
Is Genetic Engineering worth the cost and effort?
Cystic fibrosis (CF) occurs with a frequency of about 1/2500 Caucasian newborns and is inherited as an autosomal recessive. A woman had an older sister die from complications of this disease. CF is not present among relatives of her husband. Both the woman and her husband have normal phenotypes. What is the chance this couple will have a CF child?
