Interested to Buy Any Domain ? << Click Here >> for more details...
what is the method used to identify your transformed cells
from the plasmid vector?
- 2 Answers
- 7614 Views
- I also Faced
- E-Mail Answers
Answer Posted / kk pandey
I)If transformant is thought to have antibiotic resistant
gene (called as Marker Gene)along with ur desired gene, then
culture those cells in a media containing that same
antibiotic.
==>Case 1:- the cell is not transformed.:-
the cell will not have the antibiotic resistant gene, hence
it wont grow (or simply die)bcoz of antibiotic in the media.
==>Case 2:-the cell is successfully transformed.:-
The cell will easily grow in media bcoz antibiotic wont
affect it as cell will resist it.(bcoz of marker gene).
-----*----
My answer is quite long but i think i will properly make u
understand.
| Is This Answer Correct ? | 8 Yes | 2 No |
Post New Answer View All Answers
How is a genetic trait determined by the genetic code within a dna molecule?
How will you differentiate alleles of a gene in the double strand of DNA?
what causes heterosexuality?
How DNA is being Transplanted?
In horses coat color is dependent upon genes as follows: A_B_ results in bay, aaB_ in black, A_bb in chestnut, aabb in liver. In addition, palimino is derived by Dd with chestnut. A palimono stallion (DdAAbb) is crossed with a palimino mare (DdAAbb). What percent of the offspring will be palimino?
What are the down sides of Genetic Engineering?
To how much extent skin color depends on genes?
Differentiate between biotechnology and bioengineering?
Cystic fibrosis (CF) occurs with a frequency of about 1/2500 Caucasian newborns and is inherited as an autosomal recessive. A woman had an older sister die from complications of this disease. CF is not present among relatives of her husband. Both the woman and her husband have normal phenotypes. What is the chance this couple will have a CF child
how was highschool like for a person diagnosed with turner's syndrome? and really difficult subjects?
Flower position, stem length, and seed shape were three traits that were studied by Mendel. Each is controlled by an independently assorting gene. If a plant that is heterozygous for all three traits was allowed to self- fertilize, what proportion of the offspring would be expected to show all three dominant phenotypes?
Has vitamin c been produced in a human naturally before?
Why do we age?
Consider two linked autosomal genes. The dominant allele C of the first gene causes cataracts of the eye, whereas its recessive allele produces normal eyes. The dominant allele P causes polydactyly (an extra finger) whereas its allele (p) produces a normal number of fingers. A man with cataracts and normal fingers marries a woman with polydactyly and normal eyes. Their son has both polydactyly and cataracts and he marries a woman with neither trait. What is the probability that their first child will have cataracts and polydactyly?
What is the chemical composition of gene?
