what happens when aminoacid reacts with edman reagent?
Answer Posted / vipul panchal
In presence of edman reagent, amino acid undergoes condensation reaction and gets cyclized.
Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
What are zymogens?
Where is csf found? What is its function?
Concerning enzymatic reactions how different, are the graphic curve of the variation of the speed of a reaction as function of substrate concentration and the curve of variation of the speed of a reaction as function of temperature?
Which elements are present in electron?
i have done M.SC. in biochemistry from india. as i have experience of pharmaceuticals in quality assurance department of 4 year.now i come to know that my degree is valid in uk.i got my comparision letter of degree from uknaric. but i m confuse that what is the next step to do for getiing job in lab? am i eligible to get directly job or as trainee than job ? or to register in ibms or what? pls tell me
How much empty space is found in globular proteins?
What happen to a denaturated enzyme regarding its functionality? How that result can be explained with the help of the lock and key model?
What factors can change the pKa value of an amino acid residue, and in which direction?
Into which two groups can the nitrogen-containing bases that form dna and rna be classified? What is the criterion used in that classification?
How do peptides react with cyanogen bromide?
What are the structures of the products of this reaction, and how are they identified?
How many elements are there in 3d-series of first transition series?
mutarotation is observed when phenol and pyridine both are present but phenol or pyridine alone cannot show mutarotation-explain why?
What is the structure of cscl