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A principal difference between prokaryotic and eukaryotic
DNA replication is
a) completely different proteins/enzymes in eukaryotes
b) multiple origins in eukaryotes
c) no requirement for topoisomerase activity in prokaryotes
d) the absence of a nucleus in prokaryotes
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Answer Posted / pooja
a)In prokaryotes HU proteins creates negative coiling and
Tus protein terminates DNA replication. Topoisomerase IV
unlinks two circular DNA duplexes. In eukaryotes PCNA
protein prevents PCNA-Rfc-Polymerase delta complex from
dissociating from template. Telomerase are required for
synthesis in end region of lagging strand of DNA. This is
because replication takes place in opposite direction and
DNA polymerase is not capable of adding nucleotides in end
region.
b)Origin of replication always takes place from OriC. This
region is A-T rich. This is because there is double bond
between A and T it would require less energy for breakdown
compared to G-C. Probability of DNA replication to initiate
depends on presence of these nucleotides.
c)HU protein creates negative supercoils in prokaryotes.
Inorder to remove these supercoiling DNA gyrase(belongs to
class topoisomerase)is required.
d)In prokaryotes nucleus is not present, genetic material
is present within the nucleoid.
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